Multi-electrode arrays (MEA) measure extracellular voltage changes that occur as neurons fire action potentials. These measurements reveal the firing patterns of individual neurons as well as the patterns of neuronal networks that exist in the cell culture. Such measurements are non-invasive and allow for repeated recordings.
Layer V Glutamatergic Neurons (BX-0350) were cultured on Axion Biosystems MEA plates for several weeks and recorded regularly. Below, a time course of the number of active electrodes, mean firing frequency, and synchrony index reveal the development of neuronal activity in Layer V Glutamatergic Neurons over several weeks in culture.